RNA sequencing Human

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Get tips on using GenCatch™ Total RNA Extraction Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized SK-BR-3

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis