Stem cell Differentiation media

Get tips on using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm to perform Cell migration / Invasion cell type - BRO

Get tips on using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm to perform Cell migration / Invasion cell type - LNCaP

Get tips on using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm to perform Cell migration / Invasion cell type - PC-3

Get tips on using CytoSelect™ 24-Well Cell Migration and Invasion Assay Combo Kit, 8 µm to perform Cell migration / Invasion cell type - SK-MEL-1

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis HeLa

Get tips on using Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit to perform Necrosis A549