siRNA / miRNA gene silencing Human HEK293

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

Get tips on using Human Candida Albicans ELISA Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media PDX mammospheres

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - UMR-106

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using PE Mouse Anti-Human CD31 Clone L133.1 to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Get tips on using PE anti-human CD111 (Nectin-1) Antibody to perform Flow cytometry Anti-bodies Human - CD111/Nectin-1

Get tips on using PE anti-human CD126 (IL-6Rα) Antibody to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.