DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using Human vWF-A2 DuoSet ELISA to perform ELISA Human - VWF-A2
Get tips on using Human PDGF-BB ELISA Kit to perform ELISA Human - PDGF-BB
Get tips on using Human PDGF-BB DuoSet ELISA to perform ELISA Human - PDGF-BB
Get tips on using Human IGF1 ELISA Kit (ab100545) to perform ELISA Human - IGF-I
Get tips on using GM-CSF Human ELISA Kit to perform ELISA Human - GM-CSF
Get tips on using Human GM-CSF DuoSet ELISA to perform ELISA Human - GM-CSF
Get tips on using Cytochrome C Human ELISA Kit to perform ELISA Human - Cytochrome C
Get tips on using Human BMP2 ELISA Kit (ab119581) to perform ELISA Human - BMP-2
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment