Live / Dead assay

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Get tips on using Anti-ATG5 (C-terminal) antibody produced in rabbit to perform Autophagy assay cell type - Rat spinal cord tissue

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - K562 human leukemia cells

Get tips on using Purified Mouse Anti-Nucleoporin p62 Clone 53/Nucleoporin p62 to perform Autophagy assay cell type - N27 dopaminergic cells

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - Human T-cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Liver tissue

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Liver