Get tips on using Monoclonal Mouse Anti-Villin (Autostainer Link 48) Clone 1D2 C3p to perform DNA Damage Assay U266 -
Get tips on using Purified Mouse Anti-E-Cadherin Clone 36/E-Cadherin (RUO) to perform Immunohistochemistry Human - E-Cadherin
Get tips on using Monoclonal Mouse Anti-Thyroid Transcription Factor (Concentrate) Clone 8G7G3/1 to perform Immunohistochemistry Human - TTF-1
Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit to perform Microarray Human - PCOS
Get tips on using Mouse Monoclonal Antibody to Lamin A/C Cat# MCA-4C4 to perform Western blotting Lamin A/C
Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Rat - TGF-beta 1
Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts
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