Get tips on using Purified Hamster Anti-Mouse CD80 to perform Flow cytometry Anti-bodies Mouse - CD80
Get tips on using FITC Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40
Get tips on using PE Hamster Anti-Mouse CD279 to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1
Get tips on using Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 568 conjugate to perform Flowcytometry Secondary Antibody - Goat Mouse Alexa Fluor 568
Get tips on using Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate to perform Flowcytometry Secondary Antibody - Goat Rabbit Alexa Fluor 488
Get tips on using Purified Hamster Anti-Mouse TCR β Chain to perform Flow cytometry Anti-bodies Mouse - TCRbeta
Get tips on using Purified NA/LE Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40
Get tips on using PerCP-Cy™5.5 Hamster Anti-Mouse CD69 to perform Flow cytometry Anti-bodies Mouse - CD69
Get tips on using PE-Cy™7 Hamster Anti-Mouse CD11c to perform Flow cytometry Anti-bodies Mouse - CD11c
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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