Immunohistochemistry Alpha smooth muscle Actin Mouse -NA-

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Get tips on using Smooth Muscle Growth Supplement (SMGS) to perform Mammalian cell culture media HCASMC

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Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

Products PromoCell Smooth Muscle Cell Growth Medium 2

Get tips on using Canine Smooth Muscle Cell Growth Medium to perform Mammalian cell culture media CnAOSMC

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Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCASMC

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Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCtASMC

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Get tips on using Monoclonal Anti-ACTA2 antibody produced in mouse to perform Western blotting Smooth muscle actin

Products Sigma-Aldrich Monoclonal Anti-ACTA2 antibody produced in mouse

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase vascular smooth muscle cells (VSMC)

Get tips on using Mouse GFR alpha-3/GDNF R alpha-3 Antibody to perform Immunohistochemistry Mouse - Gfrα3

Products R&D system, Minneapolis, MN, USA Mouse GFR alpha-3/GDNF R alpha-3 Antibody

Get tips on using Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody to perform Immunohistochemistry Mouse - Gfrα2

Products R&D system, Minneapolis, MN, USA Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human aortic smooth muscle cells

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