Immunohistochemistry Vimentin Rabbit Human / mouse

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin

Get tips on using Recombinant Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) to perform Flow cytometry Anti-bodies Human - Vimentin

Get tips on using OSTEOPONTIN (O-17) ANTI-HUMAN RABBIT IGG AFFINITY PURIFY to perform Immunohistochemistry Mouse - Spp1/OPN

Get tips on using Recombinant Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547) to perform Western blotting Vimentin

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Rabbit Anti-Human CHK2 (NT) Affinity Purified pAb to perform Immunohistochemistry chk2 - Rabbit IgG Human -NA-

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-

Get tips on using Rabbit anti-calretinin to perform Immunohistochemistry Mouse - Calb2

Get tips on using Insulin (C27C9) Rabbit mAb #3014 to perform Immunohistochemistry Mouse - Insulin