CRISPR Rat Deletion AR42J

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1

Get tips on using pLV-EF1a-IRES-Puro to perform CRISPR Mouse - Deletion L929 Ppm1b

Get tips on using pCas-Guide-EF1a-GFP to perform CRISPR Mouse - Deletion αT3 Stim2

Get tips on using pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W to perform CRISPR Human - Deletion KAT2A

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion MGAT1

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion PKR

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion AURKB

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion ATM