ChIP Anti-bodies

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using CtIP antibody (mAb) to perform ChIP Anti-bodies CtIP/BCL11A

Get tips on using Mre11 Antibody #4895 to perform ChIP Anti-bodies MRE11

Get tips on using ChIPAb+™ HDAC1 Antibody, rabbit polyclonal to perform ChIP Anti-bodies HDAC1

Get tips on using Histone H2A.X antibody (pAb) to perform ChIP Anti-bodies γH2AX

Get tips on using Histone H4K20me1 antibody (mAb) to perform ChIP Anti-bodies H4K20me1

Get tips on using Histone H3K36me3 antibody (mAb) to perform ChIP Anti-bodies H3K36me3

Get tips on using Histone H3K9me3 antibody (mAb) to perform ChIP Anti-bodies H3K9me3

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K27me1

Get tips on using Histone H3K27ac antibody (pAb) to perform ChIP Anti-bodies H3K27ac