Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform AAA for reviews

Get tips on using Rat Angiopoietin 2,ANG-2 ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

Get tips on using Rat BMP-2 PicoKine™ ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using Rat Angiopoietin-2 PicoKine™ ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using Rat Lipocalin-2/NGAL ELISA Kit (Colorimetric) to perform ELISA Rat - NGAL/LCN2

Get tips on using Rat Lipocalin-2 ELISA Kit (NGAL) (ab119602) to perform ELISA Rat - NGAL/LCN2

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Rat Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Rat - NGAL/LCN2