siRNA / miRNA gene silencing Human SUIT-2

Get tips on using Human MMP-1 Antibody to perform Western blotting MMP1

Get tips on using Human GDNF DuoSet ELISA to perform ELISA Rat - GDNF

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Human VEGF PicoKine™ ELISA Kit to perform ELISA Human - VEGF

Get tips on using Human Sonic Hedgehog ELISA Kit (ab100639) to perform ELISA Human - ShhN

Get tips on using Human SDC1 / Syndecan-1 ELISA Kit to perform ELISA Human - SDC1

Get tips on using Human Prolactin PicoKine™ ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Human - OPN

Get tips on using Human MCP-1 ELISA Kit (ab179886) to perform ELISA Human - MCP1

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO