Protein expression and purification Mammalian cells

Get tips on using Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029) to perform Protein Ladder Prestained

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Human Adrenal glands

Get tips on using Wilms' Tumor 1 (WT1) Protein, Clone 6F-H2 to perform Immunohistochemistry Wilms Tumor 1 (WT1) - Rabbit Mouse -NA-

Get tips on using Anti-TATA binding protein TBP antibody [58C9] - Loading Control (ab61411) to perform Western blotting TBP

Get tips on using heat shock protein family A (Hsp70) member 5 to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) HSPA5 (GRP78)

Get tips on using Ni-NTA Superflow (100 ml) to perform Protein tag Purification of His-tagged proteins

Get tips on using SuperFect Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat hepatic stellate cells