siRNA / miRNA gene silencing Human COV-434

Get tips on using Human Lipocalin-2 ELISA Kit (NGAL) (ab119600) to perform ELISA Human - NGAL/LCN2

Get tips on using Human Lipocalin-2/NGAL Quantikine ELISA Kit to perform ELISA Human - NGAL/LCN2

Get tips on using Human IGF-1 PicoKine™ ELISA Kit to perform ELISA Human - IGF-I

Get tips on using Human BMP-2 PicoKine™ ELISA Kit to perform ELISA Human - BMP-2

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Human ICAM-1/CD54 Antibody to perform Western blotting ICAM-1

Get tips on using MagniSort™ Human T cell Enrichment Kit to perform Cell Isolation Human T cells

Get tips on using EasySep™ Human T Cell Enrichment Kit to perform Cell Isolation Human T cells

Get tips on using EasySep™ Human T Cell Isolation Kit to perform Cell Isolation Human T cells