rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using VEGF Receptor 2 (55B11) Rabbit mAb #2479 to perform Western blotting VEGF

Get tips on using RNeasy PowerPlant Kit (50) to perform RNA isolation / purification Plants - Seeds

Get tips on using RNeasy PowerWater Kit (50) to perform RNA isolation / purification Water samples

Get tips on using Rat Angiopoietin-2 PicoKine™ ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using CD31-FITC, 5.6E, 2 mL, ASR to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Get tips on using Rat Lipocalin-2/NGAL ELISA Kit (Colorimetric) to perform ELISA Rat - NGAL/LCN2

Get tips on using Rat Lipocalin-2 ELISA Kit (NGAL) (ab119602) to perform ELISA Rat - NGAL/LCN2

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using STEMdiff™ APEL™ 2 Medium to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)