rna-isolation-purification-tissue-mouse-small-intestine

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Neisseria gonorrhoeae

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using RNeasy PowerMicrobiome Kit (50) to perform RNA isolation / purification Viral - SARS-CoV-2

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized OSC-C2