CRISPR Mouse Deletion NIH 3T3

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Get tips on using VetMAX™ West Nile Virus Kit to perform Cell Culture Contamination Detection Kit Virus

Products Thermo Fisher Scientific VetMAX™ West Nile Virus Kit

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type NIT-1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - NIT-1

Products Enzo Life Sciences CYTO-ID® Autophagy detection kit

Get tips on using Hoechst 33342 to perform Necrosis MIA PaCa-2

Products Thermo Fisher Scientific Hoechst 33342

Get tips on using Hoechst 33342 to perform DNA quantification Human - HeLa

Products Thermo Fisher Scientific Hoechst 33342

Get tips on using bisBenzimide H 33342 trihydrochloride to perform Apoptosis assay cell type - OV2008

Products Sigma-Aldrich bisBenzimide H 33342 trihydrochloride

Get tips on using bisBenzimide H 33342 trihydrochloride to perform Apoptosis assay cell type - OVCAR-3

Products Sigma-Aldrich bisBenzimide H 33342 trihydrochloride

Get tips on using Phospho-Jak1 (Tyr1034/1035) Antibody #3331 to perform Western blotting Jak1

Products Cell Signaling Technology Phospho-Jak1 (Tyr1034/1035) Antibody #3331

Get tips on using MouseTRAP™ (TRAcP 5b) ELISA to perform Acid phosphatase assay cell type - murine macrophage cells

Products Immunodiagnosticsystems (ids) MouseTRAP™ (TRAcP 5b) ELISA

Get tips on using PageRuler™ Prestained NIR Protein Ladder to perform Protein Ladder Prestained

Products Thermo Fisher Scientific PageRuler™ Prestained NIR Protein Ladder

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