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PCR Methylation specific PCR

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Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri

Products Thermo Fisher Scientific PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA
pFR2 Product

Get tips on using pFR2 to perform Protein Expression Prokaryotic cells - E. coli ferulic acid

Products Maurizio Ruzzi, Department for Innovation in Biological, Agro-Fo pFR2
pCW-Cas9 Product

Get tips on using pCW-Cas9 to perform CRISPR Human - Repression ENII-CP/X

Products Addgene pCW-Cas9

Get tips on using pcDNA-dCas9-VP64 to perform CRISPR Human - Activation REPRIMO

Products Addgene pcDNA-dCas9-VP64

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PC-7

Products Dharmacon ON-TARGETplus Human PCSK7 (9159) siRNA - Individual

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine primary airway epithelial cell

Get tips on using Monoclonal Mouse Anti-Human Progesterone Receptor (Concentrate) Clone PgR 1294 to perform Immunohistochemistry Human - PR

Products Agilent Technologies Monoclonal Mouse Anti-Human Progesterone Receptor (Concentrate) Clone PgR 1294

Get tips on using pTrcHis-TOPO to perform Protein Expression Prokaryotic cells - E. coli Murine BCO2

Products Johannes von Lintig, Department of Pharmacology, Case Western Re pTrcHis-TOPO

Get tips on using pcDNA-dCas9-p300 Core to perform CRISPR Human - Activation MASPIN

Products Addgene pcDNA-dCas9-p300 Core

Get tips on using pCas-Guide-EF1a-GFP to perform CRISPR Mouse - Deletion αT3 Stim2

Products Addgene pCas-Guide-EF1a-GFP

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