Protein Expression Eukaryotic cells lettuce leaves

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Whole Rat Genome Microarray Kit, 4x44K to perform Microarray Gene expression arrays - Rat chorid plexus Cyanine 3

Get tips on using GeneChip™ Rat Genome 230 2.0 Array to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Get tips on using GeneChip™ Hybridization, Wash, and Stain Kit to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Get tips on using Streptavidin-R-PE to perform Protein tag Detection of biotinylated proteins

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,