DNA quantification Mouse

Get tips on using DNeasy Plant Pro Kit (250) to perform DNA isolation / purification Plants - Mycelial mats

Get tips on using DNeasy PowerClean Pro Cleanup Kit (50) to perform DNA isolation / purification Water samples

Get tips on using Comet Assay to perform DNA Damage Assay A-375

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - mouse iPSC

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - mouse keratinocytes

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform DNA Damage Assay Saos-2

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2