siRNA / miRNA gene silencing Human RWPE

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Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Mammalian cells Human gingival epithelial cells

Get tips on using Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO) to perform Immunohistochemistry Human - MLH1

Products BD Biosciences Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO)

Get tips on using Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441 to perform Immunohistochemistry Human - AR

Products Agilent Technologies Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8 to perform Immunohistochemistry Human - CK20

Products Agilent Technologies Monoclonal Mouse Anti-Human Cytokeratin 20 (Concentrate) Clone Ks20.8

Get tips on using Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit to perform ELISA Human - ShhN

Products R&D Systems Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

Get tips on using Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897) to perform ELISA Human - RBP4

Products Abcam Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)

Get tips on using Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA to perform ELISA Human - NRG1

Products R&D Systems Human NRG1-beta 1/HRG1-beta 1 DuoSet ELISA

Get tips on using Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit to perform ELISA Human - FABP2

Products Sigma-Aldrich Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit

Get tips on using Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit to perform ELISA Human - BRCA2

Products MyBioSource.com Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

Proteins Immunohistochemistry Human GR/glucocorticoid receptor

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