CRISPR Rat Deletion PC12

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Rat - PC12

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1

Get tips on using pLV-EF1a-IRES-Puro to perform CRISPR Mouse - Deletion L929 Ppm1b

Get tips on using pCas-Guide-EF1a-GFP to perform CRISPR Mouse - Deletion αT3 Stim2

Get tips on using pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W to perform CRISPR Human - Deletion KAT2A

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Deletion MGAT1

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion PKR

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion AURKB