shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using siGENOME Human AP1G1 (164) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa γ1-adaptin/AP1G1

Get tips on using FoxP3 Antibody, anti-mouse, PE, REAfinity™ to perform Flow cytometry Anti-bodies Mouse - FOXP3

Get tips on using Purified anti-mouse CD115 (CSF-1R) Antibody to perform Flow cytometry Anti-bodies Mouse - CD115

Get tips on using Mouse Sonic Hedgehog/Shh DuoSet ELISA to perform ELISA Mouse - ShhN

Get tips on using Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458) to perform ELISA Mouse - MPO

Get tips on using pro-MMP-9 Mouse ELISA Kit to perform ELISA Mouse - MMP9

Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Mouse GDNF PicoKine™ ELISA Kit to perform ELISA Mouse - GDNF