shRNA gene silencing Mouse RGC-5

Get tips on using Low Input Quick Amp Labeling Kits to perform Microarray RNA amplification & Labeling - Fish fundulus heteroclitus Cyanine-3 / Cyanine-5

Get tips on using Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Amino Allyl MessageAmp™ II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Fish fundulus heteroclitus Cyanine-3 / Cyanine-5

Get tips on using Amino Allyl MessageAmp™ II aRNA Amplification Kit to perform RNA amplification & labeling Fish - Total RNA, Fundulus heteroclitus Cyanine 3 & 5

Get tips on using Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit to perform Cell cycle assay human - OVCAR-5

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using SQSTM1/p62 Antibody #5114 to perform Immunohistochemistry Mouse - p62

Get tips on using PCNA Antibody (PC10): sc-56 to perform Immunohistochemistry Mouse - PCNA

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Mouse - C2C12 myogenin