Protein isolation Bacteria

Get tips on using pcDNA-nGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pcDNA-oGP1 to perform Protein Expression Eukaryotic cells - HEK293 GP1

Get tips on using pMT3-RhoGC to perform Protein Expression Eukaryotic cells - HEK293 RhoGC

Get tips on using pONE-40A to perform Protein Expression Eukaryotic cells - HEK293 MBP

Get tips on using PL_Mm Ppp1r15a to perform Protein Expression Eukaryotic cells - CHO Ppp1r15a

Get tips on using PL_Mm P4hb to perform Protein Expression Eukaryotic cells - CHO P4hb

Get tips on using pGS-CD62L to perform Protein Expression Eukaryotic cells - CHO CD62L

Get tips on using pMH3-rFVII to perform Protein Expression Eukaryotic cells - CHO rFVII

Get tips on using 2-D Quant Kit to perform Protein quantification Colorimetric method

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.