siRNA / RNAi /miRNA transfection Mouse

Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using Targefect-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Kidney

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Brain