Microarray Gene expression arrays Human whole blood cells

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pMHL_2002-bfloGFPa1 to perform Protein Expression Eukaryotic cells - T. pseudonana IbpA DR2 antigen from Histophilus somni

Get tips on using pPICZαB/α-amylase to perform Protein Expression Eukaryotic cells - P. pastoris G. stearothermophilus SR74 α-amylase

Get tips on using pTip-QC2-gi_21222831 to perform Protein Expression Prokaryotic cells - R. erythropolis putative merR-family transcriptional regulator

Get tips on using pTip-QC2-gi_21222214 to perform Protein Expression Prokaryotic cells - R. erythropolis putative araC-family transcriptional regulator

Get tips on using rMaj-pCHH-B to perform Protein Expression Prokaryotic cells - E. coli M. japonicus CHH-like peptide

Get tips on using pTY- α-amylase to perform Protein Expression Prokaryotic cells - E. coli Pyrococcus woesei Hyperthermophile α-Amylase

Get tips on using pST44-yAda3Δ2HIS-yAda2Δ1-yGcn5 to perform Protein Expression Prokaryotic cells - E. coli Ada2/Ada3/Gcn5 complex

Get tips on using pHIL‐S1‐opt‐RABV‐G to perform Protein Expression Eukaryotic cells - P. pastoris opt‐RABV‐G