siRNA / miRNA gene silencing Mouse Glomerular mesangial cells

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Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse dorsal skin Biotin

Products Thermo Fisher Scientific GeneChip® HT 3' IVT PLUS Reagent Kit

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - Aspc-1

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - Bxpc-3

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

Get tips on using Monoclonal Mouse Anti-Human p53 Protein (Dako Omnis) Clone DO-7 to perform Immunohistochemistry Human - p53

Products Agilent Technologies Monoclonal Mouse Anti-Human p53 Protein (Dako Omnis) Clone DO-7

Get tips on using Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 to perform Immunohistochemistry Human - E-Cadherin

Products Agilent Technologies Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Concentrate) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

Products Agilent Technologies Monoclonal Mouse Anti-Human Ki-67 Antigen (Concentrate) Clone MIB-1

Get tips on using Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO) to perform Western blotting ZO-1

Products BD Biosciences Purified Mouse Anti-Human ZO-1 Clone 1/ZO-1 (RUO)

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

Products Zymo Research EZ DNA Methylation kit

Get tips on using BRCA2 rtu elisa kit :: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) RTU ELISA Kit to perform ELISA Mouse - BRCA2

Products MyBioSource.com BRCA2 rtu elisa kit :: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) RTU ELISA Kit

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Mouse Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

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