Microarray Gene expression arrays Mouse brain tissue

Get tips on using Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Mouse Horseradish peroxidase

Get tips on using FITC Mouse Anti-Human CD51/CD61 to perform Flow cytometry Anti-bodies Human - CD51

Get tips on using PE Mouse Anti-Human CD51/CD61 to perform Flow cytometry Anti-bodies Human - CD51

Get tips on using APC-H7 Mouse Anti-Human CD43 to perform Flow cytometry Anti-bodies Human - CD43

Get tips on using APC-H7 Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using PE-CF594 Mouse Anti-Human FoxP3 to perform Flow cytometry Anti-bodies Human - FOXP3

Get tips on using CD11b Antibody, anti-human/mouse, FITC to perform Flow cytometry Anti-bodies Human - CD11b

Get tips on using APC-H7 Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

Get tips on using EpiCult™-B Mouse Medium Kit to perform Stem cell culture media Murine mammospheres

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.