Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human breast tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human lung tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human placental tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human brain tissue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human aorta tissue
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - Rat Kidney
Get tips on using PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit to perform Cell Culture Contamination Detection Kit Mycoplasma
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Thyroid gland
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