Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat dorsal root ganglion neurons
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.
Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat cardiomyocytes
Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat MSC
Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Rat saphenous arteries Biotin
Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
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