siRNA / miRNA gene silencing Rat INS-1

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - NRK52E

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Rat - NRK52E

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Liver

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Rat - Pancreas

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Rat - Pancreas

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain

Get tips on using Human SCF ELISA Kit (ab100636) to perform ELISA Rat - SC

Get tips on using HSP70 High Sensitivity ELISA kit to perform ELISA Rat - HSP70

Get tips on using Total BDNF Quantikine ELISA Kit to perform ELISA Rat - BDNF

RNA quantification for appropriate concentration and quality (260/280 ratio) is an important step before downstream analysis (including sequencing, RT-qPCR, etc.). Having insufficient RNA quantities or a high salt or phenol in the RNA product can lead to variable or irreproducible downstream results. The various methods used for RNA quantification include: 1. UV spectrophotometric (challenges include: low sensitivity, cannot distinguish between nucleic acid species), 2. Fluorescence-based (challenges include: requires standards, cannot measure amplifiability, not sequence-specific), and 3. RT-PCR (challenges include: requires standards, time-intensive, costly). In order to overcome these challenges, and also to ensure the proper quantification and quality control for RNA product, it is important to use at least two or more methods in order to discard any inconsistencies. Using standards for calibrations increases the sensitivity range for RNA detention (fluorescence- and RT-PCR-based methods). When using RT- PCR, it is important to choose correct primers, aligning to the desired site on the template and of appropriate product length, along with positive, negative and loading controls. It is also important to have at least two primer pairs in order to confirm results.