protein-isolation-tissue-mouse-lung-tissue

Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Mouse - OPN

Get tips on using Mouse Total MMP-9 Quantikine ELISA Kit to perform ELISA Mouse - MMP9

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using GFAP (GA5) Mouse mAb #3670 to perform Immunohistochemistry Rat - GFAP

Get tips on using Human/Mouse NLRP3/NALP3 Antibody to perform Western blotting NLRP3

Get tips on using Human/Mouse/Rat SOX2 Antibody to perform Western blotting SOX2

Get tips on using PCNA (PC10) Mouse mAb #2586 to perform Western blotting PCNA

Get tips on using Mouse Rat Estradiol ELISA Kit to perform ELISA Rat - Estradiol

Get tips on using Mouse GDNF ELISA Kit (ab171178) to perform ELISA Human - GDNF