When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5 to perform Flow cytometry Anti-bodies Human - CD23
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary arterial smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human umbilical artery smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment