Site Directed Mutagenesis (SDM) Human Insertion IMR-32

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - THP-1

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - HEK 293

Get tips on using Silencer® Select_HDAC8 siRNA to perform siRNA / miRNA gene silencing Human - UCC HDAC8

Get tips on using Silencer® Select_SPRY2 siRNA to perform siRNA / miRNA gene silencing Human - RMS SPRY2

Get tips on using Silencer® Select_ MYD88 to perform siRNA / miRNA gene silencing Human - A375 MYD88

Get tips on using Silencer® Select_ TRIF to perform siRNA / miRNA gene silencing Human - A375 TRIF

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Seminal vesicles

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - PANC-1