dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Mono-Mac-6

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-361

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized CAMA-1

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized SH-SY5Y

Get tips on using PureHelix™ RNA Extraction Solution to perform RNA isolation / purification Cells - immortalized HT-1080

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized Caco-2

Get tips on using QIAGEN Plasmid Plus Midi Kit (25) to perform DNA isolation / purification Plasmid purification

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Human - Fibroblast cell lines

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.