Site Directed Mutagenesis (SDM) Hamster Point mutation BHK-21

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized BHK-21

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media BHK-21

Get tips on using NP40 Cell Lysis Buffer to perform Protein isolation Mammalian cells - BHK-21

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media BHK-21

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media BHK-21

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - BHK-21

Get tips on using CelLytic™ NuCLEAR™ Extraction Kit to perform Protein isolation Mammalian cells - BHK-21

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - BHK-21