RNA quantification

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - SKBR-3

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Human - MCF-7

Get tips on using ScriptSeq Complete Gold Kit (Epidemiology) to perform RNA sequencing Human - SH-SY5Y

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.