DNA transfection Mammalian cells Immortalized cell lines

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using LightCycler® 480 SYBR Green I Master to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Brilliant II SYBR Green qPCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using GeneRead DNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using FlexiGene DNA Kit (250) to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Yeast DNA Extraction Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using GoTaq® DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA