RNA sequencing Human

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Get tips on using MojoSort™ Human Pan Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using Slan (M-DC8)+ Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using MagniSort™ Human pan-Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using Dynabeads™ Untouched™ Human Monocytes Kit to perform Cell Isolation Monocyte

Get tips on using EasySep™ Direct Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Get tips on using NLRP3/NALP3 (human) monoclonal antibody (Nalpy3-b) to perform Western blotting NLRP3

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using PE anti-human CD135 (Flt-3/Flk-2) Antibody to perform Flow cytometry Anti-bodies Human - CD135