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DNA isolation / purification Bacteria

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Protein tag Purification of phosphorylated proteins Experiment

Proteins Protein tag Purification of phosphorylated proteins

DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded Experiment

DNA DNA quantification Human Colorectal aenocarenoma (SW48) - paraffin embeded

PCR Quantitative real-time PCR - Fish species DNA Experiment

DNA PCR Quantitative real-time PCR Fish species DNA

Purification of extracellular vesicles Exosomes - Plasma Experiment

Cellular assays Purification of extracellular vesicles Exosomes Plasma

EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit Product

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - HepG2 FHIT

Products Fisher Scientific EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit

EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit Product

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Hep3B SFRP3

Products Fisher Scientific EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit

Some help with RNA isolation using Trizol Discussion

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

Pan Monocyte Isolation Kit, human Product

Get tips on using Pan Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Products Miltenyibiotec Pan Monocyte Isolation Kit, human

Classical Monocyte Isolation Kit, human Product

Get tips on using Classical Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Products Miltenyibiotec Classical Monocyte Isolation Kit, human

DNA Damage Assay Human bronchial epithelial cells (hBE) - Experiment

Cellular assays DNA Damage Assay Human bronchial epithelial cells (hBE)

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