Stabilization of DNA

Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Immortalized cell lines iPSC

Get tips on using MagNA Pure Compact Nucleic Acid Isolation Kit I to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Get tips on using Q5® Hot Start High-Fidelity 2X Master Mix to perform PCR Hot start PCR - Mammalian DNA

Get tips on using Penta·His Biotin Conjugate to perform Protein tag Detection of His-tagged proteins

Get tips on using exoEasy Maxi Kit (20) to perform Purification of extracellular vesicles Exosomes - Plasma

Get tips on using exoRNeasy Midi Kit (50) to perform Purification of extracellular vesicles Exosomes - Plasma

Get tips on using PhosphoProtein Purification Kit (6) to perform Protein tag Purification of phosphorylated proteins

Get tips on using DyeEx 2.0 Spin Kit (250) to perform Removal of unincorporated dye terminators

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA

Isolating RNA from tissues and paraffin embeded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the intigrity of RNA