Protein expression and purification Bacteria DH10Bac™

Get tips on using EZ-ChIP™ to perform ChIP Human - HUVEC

Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4

Get tips on using EZ-ChIP™ to perform ChIP Rat - Mesenteric arteries

Get tips on using EZ-ChIP™ to perform ChIP Human - HUH-7

Get tips on using EZ-ChIP™ to perform ChIP Human - Caco-2

Get tips on using EZ-ChIP™ to perform ChIP Human - HEK 293

Get tips on using EZ-ChIP™ to perform ChIP Human - PANC-1

Get tips on using pwPICZalpha-DT390-bi-pIL-2-Non-N-Gly to perform Protein Expression Eukaryotic cells - P. pastoris Porcine IL-2 fusion toxins

Get tips on using STEMdiff™ Pancreatic Progenitor Kit to perform Stem cell Differentiation media Differentiation of Human hESCs into pancreatic progenitors

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.