Site Directed Mutagenesis (SDM) Human

Get tips on using Anti-Notch1 antibody (ab27526) to perform Immunohistochemistry Human - Notch1

Get tips on using MLH1 antibody [G168-15] to perform Immunohistochemistry Human - MLH1

Get tips on using Anti-CRISP3 antibody (ab105951) to perform Immunohistochemistry Human - CRISP3

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - AGS

Get tips on using Re-ChIP-IT® to perform ChIP Human - LCL

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using Silencer select_ObR siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 ObR(leptin receptor/LEPR)

Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Human WA09 hESC