rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Primary cells Bone marrow mononuclear cells

Get tips on using Gentra Puregene Buccal Cell Kit (100) to perform DNA isolation / purification Cells - Primary cells Buccal cells

Get tips on using Recombinant Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) to perform Western blotting integrin alpha2

Get tips on using β-2-Microglobulin Antibody (BBM.1): sc-13565 to perform Western blotting β₂ microglobulin

Get tips on using Recombinant Anti-beta 2 Microglobulin antibody [EP2978Y] (ab75853) to perform Western blotting β₂ microglobulin

Get tips on using Mouse Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Mouse - NGAL/LCN2

Get tips on using Human Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Human - NGAL/LCN2

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Rat Artery / Aorta

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat brain tissue