Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat spleen tissue
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue
Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Rat Adrenal glands
Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - rat heart tissue
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - BV-2
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using QIAamp UltraSens Virus Kit (250) to perform RNA isolation / purification Supernatant from cell cultures
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - human kidney tissue
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