DNA isolation / purification Cells

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using REPLI-g Mitochondrial DNA Kit (25) to perform Whole Genome Amplification Human

Get tips on using LightCycler® FastStart DNA Master SYBR Green I to perform PCR Quantitative real-time PCR - Mammalian DNA

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform