Site Directed Mutagenesis (SDM) Hamster Point mutation BHK-21

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - BHK-21

Get tips on using Rat / Mouse FGF-21 ELISA Kit to perform ELISA Rat - FGF-21

Get tips on using Fibroblast Growth Factor 21 Mouse/Rat ELISA to perform ELISA Rat - FGF-21

Get tips on using Mouse/Rat FGF-21 Quantikine ELISA Kit to perform ELISA Rat - FGF-21

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using Anti-podoplanin, Clone RTD4E10 to perform Immunohistochemistry Podoplanin - Hamster Mouse -NA-

Get tips on using pRSFDuet™-1 DNA - Novagen to perform CRISPR Hamster - Deletion CHO-K1 COSMC

Get tips on using pRSFDuet™-1 DNA - Novagen to perform CRISPR Hamster - Deletion CHO-K1 FUT8

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα