DNA transfection Mammalian cells Immortalized cell lines

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - A549

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - K562

Get tips on using DMEM to perform Mammalian cell culture media HFLS

Get tips on using IMDM to perform Mammalian cell culture media KG1

Get tips on using IMDM to perform Mammalian cell culture media U937

Get tips on using Q5® Hot Start High-Fidelity 2X Master Mix to perform PCR Hot start PCR - Mammalian DNA

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Fluorescent High Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Low Range DNA Ruler Plus to perform DNA Ladder Low Range